Journal: Scientific Reports

Article Title: Human platelet lysate combined with mesenchymal stem cells pretreated with platelet lysate improved cardiac function in rats with myocardial infarction

doi: 10.1038/s41598-024-79050-6

Figure Lengend Snippet: ( A ): Sample micrograph of the left ventricle of one animal from each group showing the effect of different treatments on the CD31 + expression area (brown color, marked by the red arrow) assessed by immunohistochemistry (IHC) in hearts four weeks after LAD occlusion. ( B ): Quantification of the CD31 + expression area (%) in study groups. *** P < 0.001 vs. sham; # P < 0.05 ## P < 0.01 ### P < 0.001 vs. Veh; && P < 0.01 vs. PMSCs; $$ P < 0.01 vs. PMSC lysate. Magnification = 400x. n = 7 in each group.

Article Snippet: The following materials were used in this study: TTC from Sigma, UK; Dulbecco’s Modified Eagle Medium (DMEM) and Fetal Bovine Serum (FBS) from Gibco, CD31 polyclonal antibody from Elabscience Biotechnology Inc., ab64261 - rabbit specific HRP/DAB (ABC) detection IHC kit from Abcam.

Techniques: Expressing, Immunohistochemistry

Journal: Plastic and Reconstructive Surgery

Article Title: Extracellular Vesicles from Adipose-Derived Stem Cells Relieve Extremity Lymphedema in Mouse Models

doi: 10.1097/PRS.0000000000010388

Figure Lengend Snippet: ADSC-EVs increase lymphangiogenesis and angiogenesis and decrease fibrosis in the lymphedema limb. ( Above , left ) Representative 40× images of sections from control and ADSC-EV–treated limbs 6 weeks after surgery with the immunofluorescence localization of LYVE-1 + and CD31 + cells. ( Above , right ) Quantification of the LYVE-1 + CD31 + overlap areas per 0.25 mm 2 (four random areas per limb) after treatment with PBS, HEK293-EVs, or ADSC-EVs. All the data are presented as the mean ± SEM, with P < 0.05 considered as significant (compared with the PBS group). Scale bars = 100 μm. ( Below , left ) Representative 20× images of limb sections from control and ADSC-EV–treated limbs 6 weeks after surgery with immunofluorescence localization of collagen 1, Hoechst, and CD31. ( Below , right ) Quantification of collagen 1–positive cells per 0.25 mm 2 (four random areas per limb) after treatment with PBS, HEK293-EVs, or ADSC-EVs. All the data are presented as the mean ± SEM, with P < 0.05 considered as significant (compared with the PBS group). Scale bars = 100 μm.

Article Snippet: The tissues were stained with Hoechst 33342 (Dojindo, Tokyo, Japan), lymphatic vessel endothelial receptor-1 (LYVE-1) polyclonal antibodies (bs-1311R-A555; Bioss Antibodies), anti-CD31 polyclonal antibodies (bs-0468R-A647; Bioss), SMAD3 polyclonal antibodies (bs-2225R-A488; Bioss), and collagen 1 polyclonal antibodies (bs-10423R-A488; Bioss).

Techniques: Control, Immunofluorescence

Journal: Biomedicines

Article Title: Sodium Accumulation and Blood Capillary Rarefaction in the Skin Predispose Spontaneously Hypertensive Rats to Salt Sensitive Hypertension

doi: 10.3390/biomedicines10020376

Figure Lengend Snippet: Representative images of skin blood and lymphatic capillaries. ( A ). Anti-CD31/PECAM-1 immunohistochemistry. The antibody stains endothelial lining of dermal blood vessels (arrows). Original magnification ×40. ( B ). Anti-podoplanin immunohistochemistry. Primary antibody highlights dermal lymphatic vessels (arrows). Original magnification ×40.

Article Snippet: For immunohistochemical analysis, tissue sections (4 μm-thick) were pre-treated by heat mediated antigen retrieval and incubated with primary rabbit polyclonal anti-CD31/PECAM-1 antibody (AP31323PU-N, Acris Antibodies GmbH, Herford, Germany, dilution 1:1000) to detect vascular endothelia, and with primary mouse monoclonal antibody anti-Podoplanin (clone LF3/B7/D5B27, NB110-96423, Novus Biologicals, Cambridge, UK, dilution 1:200) to detect lymphatic vessels.

Techniques: Immunohistochemistry